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Complete probe sequences and the identity of the genes detected by the reference probes are available online. In addition, ten reference probes are included that detect autosomal chromosomal locations. One probe is included for exon 1b, an alternative first exon that is not present in BRCA1 transcript variant 1, and two probes detect sequences located 4.7 kb and 0.8 kb upstream of the BRCA1 gene. Three probes are present for exon 24 and two probes for exon 16. Three probes are present for exon 13, which is frequently deleted or duplicated (Hogervorst et al. Eight probes are present for exon 11 (3426 nt long). This includes 38 probes for the BRCA1 gene region.Īt least one MLPA probe is present for each exon in the major BRCA1 transcript variant 1. Probemix content: The SALSA MLPA probemix P002-D1 BRCA1 contains 48 MLPA probes with amplification products between 130 and 469 nucleotides (nt). 1997), while in a Danish cohort of HBOC patients the prevalence was 3.8% (Thomassen et al. 2003), in the Netherlands 27%-36% (Hogervorst et al. For example in Italian HBOC families the prevalence is 23% (Montagna et al. Large genomic rearrangements (LGRs) in BRCA1 may account for up to 25% of all disease-causing mutations, dependent on the population (Smith et al. These copy number changes are usually missed by amplicon-based sequencing analysis (Sanger sequencing or Next Generation Sequencing), but can be detected by the MLPA technique and hence MLPA complements sequence analysis of the BRCA1 gene. Deletions and duplications of complete exons in the BRCA1 gene are the second most common cause of defects in the BRCA1 gene. The great majority of germline defects in the BRCA1 gene are point mutations that can be detected by sequence analysis. In addition, mutations in the BRCA1 and BRCA2 genes account for around 15% of ovarian cancers overall. Mutations in the BRCA1 and BRCA2 genes account for about 20 to 25% of hereditary breast cancers and about 5 to 10% of all breast cancers. Features characteristic of hereditary, versus sporadic, breast cancer are: younger age at diagnosis, frequent bilateral disease, and more frequent occurrence of disease among male relatives. Clinical background: Germline defects in the BRCA1 gene are the most frequent cause of a hereditary predisposition to breast cancer. 2 To be used in combination with a SALSA MLPA Reagent Kit and Coffalyser.Net analysis software. In all other countries, the product is for research use only (RUO). 1 Please note that this probemix is for in vitro diagnostic (IVD) use in the countries specified at the end of the product description. ![]() ![]() This device is not intended to be used for standalone diagnostic purposes, pre-implantation or prenatal testing, population screening, or for the detection of, or screening for, acquired or somatic genetic aberrations, e.g from DNA extracted from formalin-fixed paraffin embedded (FFPE) or fresh tumour materials. ![]() The results of this test should be interpreted by a clinical molecular geneticist or equivalent. It is therefore recommended to use this SALSA MLPA probemix in combination with sequence analysis.Īssay results are intended to be used in conjunction with other clinical and diagnostic findings, consistent with professional standards of practice, including confirmation by alternative methods, clinical genetic evaluation, and counselling, as appropriate. Most defects in the BRCA1 gene are point mutations, none of which will be detected by MLPA. In particular, CNVs detected by only a single probe always require confirmation by another method. P002 BRCA1 is intended to confirm a potential cause for and clinical diagnosis of hereditary breast and ovarian cancer (HBOC) syndrome and for molecular genetic testing of at-risk family members.Ĭopy number variations (CNVs) detected with the P002 BRCA1 probemix should be confirmed with the SALSA MLPA P087 BRCA1 Confirmation probemix or a different technique. Intended purpose: The SALSA MLPA Probemix P002 BRCA1 is an in vitro diagnostic (IVD) 1 or research use only (RUO) semi-quantitative assay 2 for the detection of deletions or duplications in the human BRCA1 gene in genomic DNA isolated from human peripheral whole blood specimens. ![]() "Then fit out a ship that will carry you to the mainland. Telemachus is powerless to do anything about it. And while they wait to see which one of them she will choose, they help themselves to her hospitality, feasting through her herds and guzzling her wine. Over food and wine they discuss the fact that Telemachus and his mother Penelope are plagued by suitors for Penelope's hand in marriage.Īll the eligible young nobles of Ithaca and the neighboring islands, assuming that Odysseus is dead, are vying for Penelope. Odysseus' son, Telemachus, does not recognize the goddess in her human form but invites the stranger in as a guest. I myself will inspire the hero's son." Athena departs to fulfill this vow.Īdopting a mortal guise, she appears at the gate of Odysseus' mansion on the island of Ithaca. "Then send your herald, Hermes, flying to Calypso. "You know very well it isn't," replies the god of gods. "And now Odysseus languishes on the lonely island of the nymph Calypso, pining for home. The Olympians know she refers to Poseidon, the Ruler of the Sea, who is off enjoying a banquet elsewhere. When he put out the eye of the giant Cyclops, he provoked the wrath of the God of Earthquakes." ![]() "Even though we all love Odysseus, he alone of the Greek heroes has been waylaid on his journey home from Troy. Grey-eyed Athena, the goddess of war, is addressing an assembly headed by Zeus, the king of gods: We are treated to a glimpse of life among the supreme gods on Mount Olympus. "Oh Goddess of Inspiration, help me sing of wily Odysseus, that master of schemes!" So Homer begins his epic, though the hero himself is still offstage. The Odyssey - Book One - Detailed Version ![]() Right click on the Trash, choose Empty the Trash, and click on Empty Trash again.Search and locate xScope in the Applications folder, then drag its icon with your mouse to the Trash icon (located at the end of the Dock), and drop it there.Open the Finder on your Mac, and click on the Applications folder on the sidebar.For the apps not installed through App Store: Below are two additional uninstall approaches you can adopt. No Delete button showing on the app icon? It means you can not uninstall xScope through Launchpad. If you want to reinstall the app, just launch App Store and install it again from there. Click Delete in the pop-up dialog to confirm the uninstallation of xScope.And click the Delete button appearing in the app icon. As the target app appears, position the pointer over its icon, then press and hold until the icon starts to jiggle.Click the Launchpad icon on the Dock, and then type xScope in the Search box.For the apps installed from Mac’s App Store: Besides, if you do not log in as an administrator user, you will be prompted to enter an administrator’s name and password when trying to delete an app by the steps below. To uninstall xScope without using 3rd party tool, you can adopt the conventional means. Manual Approaches to Uninstall xScope on Mac Osx Uninstaller is featured with intuitive interface, streamlined process and powerful engine, which enable all-level users to easily and fully delet xScope from the Mac. The whole process may last less than one minute to complete. Once the removal is complete, click Back to Interface button to re-scan your Mac. > Review the components belonging to xScope that are scanned out, click Complete Uninstall button, and then click Yes in pop-up dialog to uninstall xScope. > Select xScope icon in the interface, and click Run Analysis button to run a scan. > Download Osx Uninstaller, install it on your Mac, and click to launch this tool. Video: How to easily & thoroughly uninstall xScope (or other Mac apps) To completely uninstall xScope and remove its vestiges, you just need to do 3 simple steps: launch > select > uninstall. Here we would like to recommend Osx Uninstaller, a lightweight yet powerful app designed for all level users to perfectly remove problematic, stubborn or malicious apps. If you do not want to dig down the system for removing xScope, you can turn to a professional uninstall tool to accomplish the task effortlessly. There are many Mac utilities that claim to help hunt down all application files and delete them for you. Perfectly Uninstall xScope within Simple Clicks (3) Launch the Activity Monitor from Dock, Launchpad or Spotlight > type xScope in the search box > select the process pertaining to xScope > click the “X” button on the upper left corner > click Quite in the pop-up dialog (if that fails, choose Force Quite).Īfter that you can carry out a clean uninstall either automatically or manually.(2)ěring xScope to the desktop, and choose Quit option from the Apple menu.(1) Right click on its icon on the Dock, and choose the Quit option.There are several options for you to exit out of xScope: Before you start the uninstallation, you are supposed to quite the app if it is running, otherwise the uninstall may fail or trigger error message. What should you do before uninstalling xScope:įirst of all, don’t rush to drag xScope to the Trash. Struggling to uninstall unneeded applications from your Mac computer? Having trouble in removing the entire application components around the system? This tutorial will show you how to effectively uninstall xScope in macOS or Mac OS X. Once you do not want to keep an app on your Mac, you can uninstall xScope by multiple methods. You can get an app from Mac’s App Store, discs or other websites, and then install it by dragging the app icon to the Applications folder or running the provided installer in the disk image or package file. There are various kinds of software that you can install on your Mac, and it is quite easy to manage those apps on macOS. ![]() Keep your distance from the Tusken Raiders and use lightsaber throw to kill them easily. Save at the start of the mission before you kill the 3 Jawas. HIGHLY MISSABLE – Due to the fact the kill count seems to reset if you save/load during the mission, and it requires more than 10 kills. ![]() Mission 2 Droid Recovery on Tatooine – Does Anybody Like Jawas? Trophy – Kill 10 Jawas with your lightsaber strike, not lightsaber throw. Mission 2 Droid Recovery on Tatooine – Easily Startled Trophy. Mission 1 Mercenary Activity on Tatooine – Docking Bay 94 Trophy – Complete this mission. Found on the 2nd floor of the docking bay, looks like C3-P0. Mission 1 Mercenary Activity on Tatooine – This is not the droid you’re looking for trophy – kill a protocol droid with your lightsaber (Strike not throw). Mission 1 Mercenary Activity on Tatooine – Smuggling Compartment Trophy – When you enter the hangar of Kyle Katarn’s Raven Claw Ship, jump behind the crates on the right for the Secret Area. Force powers – concentrate on upgrading Heal and Lightning powers first. (Those are all the online trophies.) Stage 2 – Campaignįollow the Roadmap below, showing you which Stage to earn the trophies in. This will earn you “ Dual of the fates”, “Winner Winner Porg Dinner”, “Bounty Hunter” and “ At Last We Will Reveal Ourselves“. A roadmap is provided in the stages below.įollow us on twitter for the latest news and giveaways.Įquip a Dual Bladed Lightsaber and win a 1 vs 2 Power Dual multiplayer match without using force powers. ![]() Achievements for Steam and Xbox are in almost all cases the same as the PlayStation 4 Trophies. This can also be considered a walkthrough and may be used for PlayStation 4, Xbox One and Steam. Welcome to the trophy guide for Star Wars Jedi Knight: Jedi Academy. Missable trophies: Yes, follow the roadmap. Game: Star Wars Jedi Knight: Jedi Academy ![]() Calculate the accounts receivable turnover ratio. Accounts Receivables Turnover Ratio Formula 25,000/ 12,500. Now we can calculate Anand’s accounts receivable turnover ratio as follows: Accounts Receivables Turnover Ratio Formula Net Credit Sales / Average accounts receivable. Average accounts receivable during the period were $23,880. Here Average accounts receivable (10,000+15000)/2. Customers returned goods invoiced at $31,400 during the year. Accounts receivable turnover is an efficiency ratio or activity ratio that measures how many times a business can turn its accounts receivable into cash. Total sales of Company B during the year ended December 31, 20X0 were $984,000. Calculate the receivables turnover ratio. Its accounts receivable at July 1, 20X9 and June 30, 20Y0 were $43,300 and $51,730 respectively. Net credit sales of Company A during the year ended June 30, 20Y0 were $644,790. Also, very high values of this ratio may not be favorable, if achieved by extremely strict credit terms since such policies may distance potential buyers thus reducing sales. However, a normal level of receivables turnover is different for different industries. ![]() Increase in accounts receivable turnover overtime generally indicates improvement in the process of cash collection on credit sales. Generally, a high value of accounts receivable turnover is favorable and lower figure may indicate inefficiency in collecting outstanding sales. AnalysisĪccounts receivable turnover measures the efficiency of a business in collecting its credit sales. The beginning and ending accounts receivable may be found on the balance sheets of the first and the last day of the accounting period.Īccounts receivable turnover is usually calculated on annual basis, however for the purpose of creating trends, it is more meaningful to calculate it on monthly or quarterly basis. The goal is to maximize sales, minimize the receivable balance, and generate a high turnover rate. So, if you have credit sales for the month that total 400,000 and the account receivable balance is, for example, 60,000, the turnover rate is 6.7. We can obtain the net credit sales figure from the income statement or from the notes to the financial statements of a company.Īverage accounts receivable figure may be calculated simply by dividing the sum of beginning and ending accounts receivable by 2. Accounts Receivable Turnover Ratio Net Credit Sales / Average Accounts Receivable. FormulaĪccounts receivable turnover is calculated using the following formula: It is an activity or efficiency ratio which estimates the number of times a business collects its average accounts receivable balance during a period. Accounts receivable turnover (or simply receivables turnover) is the ratio of net credit sales of a business to its average accounts receivable during a given period, usually a year. ![]() What happens when endpoints fall out of compliance? Is it game over and the organization must start anew? Not necessarily. Having this data is incredibly useful, especially when centrally managing it through a SIEM which can sort and collate it into reports that provide IT and Security teams with a wealth of granular insight into where compliance currently stands – and further helps them to identify where opportunities exist to remediate issues and/or develop actionable plans based upon data findings when moving forward in complying with regulations. Okay, so maybe extra noise isn’t generally helpful, but in this case, the “additional noise” generated refers to the amount of data that is being collected on endpoint health statuses for all devices in your fleet. Armed with this valuable data, compliance levels can be measured and proactive workflows may be executed to ensure endpoints remain compliant and/or non-compliant devices are remediated, bringing them back into the fold before threats can be exploited. So, how are organizations expected to remain compliant while utilizing on/off-premises solutions and perform business operations locally and/or remotely using Mac?Įnter Compliance Reporter from Jamf, the security monitoring tools exclusively designed for macOS, which streams data in real-time, centrally collecting it within Security Information and Event Management (SIEM) solutions, collected logs or data lakes, allowing IT & Security teams to perform analysis that identifies security threats that pose risks of compliance exceptions. If you’re a business operating within the borders of regulation, you’ll want to make certain that you’re coloring within the lines of each of these requirements lest you be found in violation of these regulations, to which there are very severe civil and/or criminal penalties to be imposed. Furthermore, each industry in most cases has its own regulatory body with its own set of needs that each organization must adhere to.Īnd no, the fun doesn’t stop there! Each country, territory, or region that an organization operates in may be subject to the unique (and often differing) regulations imposed by the respective locale’s regulatory bodies. Each entity is subject to regulatory controls, limitations and requirements as to how data is collected, stored, managed, accessed, disseminated and disposed of. What do these industries have in common? Well, when it comes to managing their IT infrastructure, the common thread is: regulation.Įach of them houses unknown numbers of businesses under their collective umbrella. Healthcare, Finance, Legal, Education and Government. Just what you always wanted Rainier to be. Maybe, as Rainier field marketer Kurt Stream asserts in the promotional video below, "this IS the beer your great-grandfather drank." Maybe the old man didn't care to interrogate his booze. Vitamin R by Burke-Gilman Brewing Company is a Lager - American Light which has a rating of 4.1 out of 5. Maybe that's how they liked beer back in the Great Depression. Nothing much to quibble with, little to overthink. And yet, a leg up in quality from its predecessor, with a relatively round malt character, copper color, and legitimate hop bite. Still present are certain subtle flavor notes of mass production: poorish head retention, a faintly metallic aftertaste. (Though Rainier reps are quick to point out that the six-pack is composed of 16-ounce "pounder" bottles, meaning you get the equivalent of eight standard bottles for that close-to-craft brew price.)Ĭlose-to-craft-but not quite-is also how we'd describe the beer itself. Meanwhile, some Washingtonians are unsure how to feel about the brewery "homecoming." (Case in point, this from our sister publication Seattle Met.) Further complicating the reunion is price point: a six-pack of Pale Mountain Ale will retail for about $12. ![]() Reps hint that if it does well, more Rainier experiments could be in the hopper. Pale Mountain Ale hits Oregon shelves next week, and Pabst plans to also distribute in Montana, Idaho, and Northern California. And unlike your Rainier tallboy, this beer is produced not in Irwindale, California, but back in Washington, at Woodinville's Redhook facility. Meet Pale Mountain Ale: a "post-Prohibition" style with Yakima Valley Fuggle and Cascade hops, representing Rainier's first new beer in 20 years. (And do we detect, on the back end, just the faintest hint of real American rust?)Īlas, the beloved brew hasn’t been made here since 2003-taking some of the fizz out of the regal R's Cascadian cachet. ![]() It is referred to as Vitamin R, and appears prominently in a. We sipped, furrowed our brows, held the caramel-hued brew to the light, then drained our glasses. This is what your great-grandpa's go-to might have tasted like-gently hopped, generally malty, and medium-bodied. The 2008 movie Twilight, set in Forks, Washington, makes frequent reference to Rainier beer. We rather diffidently cracked open a few of these brand-new 16-oz pounders (nostalgia size!) from Washington's homegrown macrobrewer. And then, of course, there’s the motorcycle. The self-made “Rainier Man.” The MFRs-“Mountain Fresh Rainiers”- hoofing it through some of ‘70s television’s weirdest commercials. ![]() There’s Mickey Rooney as a Mountie, pouring the stuff into his wife’s cleavage. (Until both were slurped up by even Bigger Beer.) For nearly 140 years-dating back to before Washington was even a state-this mildly cornbread-y macro has permeated Pacific Northwest culture. |
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